A Method to Constrain Genome-Scale Models with 13C Labeling Data
Fig 5
Relative effect of constraints.
Confidence intervals for pentose phosphate pathway (left panel) and glycolysis (right panel) fluxes calculated using FBA constrained by measured extracellular fluxes (through Flux Variability Analysis, FVA [64], in red), for FBA with constraints derived from the two-scale approximation through the “Limit flux to core” step in Fig 3 (FVA, in grey), and for 2S-13C MFA derived through Eqs 16–23 are shown (blue). Constraints induced by the two-scale approximation are not strong, hence justifying the use of this approximation. However, constraints induced by the 13C labeling data are dominant. A similar pattern can be observed in S13 Fig.