Dynamic Allostery of the Catabolite Activator Protein Revealed by Interatomic Forces
Fig 6
Glu58 and Arg87 minimal distance decreases upon activation.
Probability distribution of the minimal distance between Glu68 and Arg87 for apo (black), cap1 (blue) and cap2 (orange) states. Both protomers were taken into account. Vertical dashed lines represent the respective values as observed in the cap2 X-ray structure (1G6N) and the range covered in the apo NMR structures (2WC2 [25]). The integration limits used in the inset for the three states (activated, intermediate and deactivated) have been determined by the two inflection points of the cap2 distribution (2.71 and 5.11 for intermediate and deactivated respectively).