Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments
Fig 3
Enrichment curves of D2-glucose labeled mice.
Best fits to the fraction of deuterium enrichment in plasma, and the percentage of label enrichment in the DNA of PBMC, splenocytes and thymocytes after seven-days of D2-glucose labeling. Dots represent individual mice. The end of label administration at day 7 is marked by a dashed vertical line. (A) Conventional normalization: measurements are normalized to the mean plasma enrichment x bg. (B) Normalized with respect to thymocytes: thymocytes were used to determine the maximum percentage of labeled DNA that cells could possibly attain (Methods), and all measured enrichments were scaled to this maximum. The raw data of the % DNA labeled in splenocytes and PBMC is the same in panel A and B but has been normalized using two different approaches.