Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments
Fig 1
Total CD4+ T cell proliferation rates estimated from D2-glucose and D2O labeling studies in humans.
Each symbol represents a different individual, horizontal lines represent the median of the estimates. (A) Proliferation rates estimated using the kinetic heterogeneity model. (B) Proliferation rates estimated after adjusting for saturation (using the multi-exponential model for the nine-week (9w) D2O and seven-day (7d) D2-glucose labeling, and the kinetic heterogeneity model for one-day (1d) D2-glucose labeling). Although correcting for the saturation of rapidly turning over subpopulations helps to bring the estimates closer (by increasing the proliferation rate estimates obtained in the nine-week D2O labeling study) significant discrepancies remain.