Dynamical Localization of DivL and PleC in the Asymmetric Division Cycle of Caulobacter crescentus: A Theoretical Investigation of Alternative Models
Fig 5
Overexpression of DivK may prevent the cell from progressing though different developmental stages.
For better comparison of mutant and wild-type distributions, the colors indicate concentration gradients from zero (blue) to maximum wild-type concentration, Cwt_max (red). For Cwt_max < Cmut_max, the red color represents all concentrations from Cwt_max to Cmut_max. For Cwt_max > Cmut_max, an additional tick between 0 and Cwt_max indicates maximum concentration in the mutant. (A) A four-fold increase in DivK synthesis (ksyn = 0.2 min-1) prevents competition between DivL and PleC kinase. DivL is not reactivated in the predivisional stage, leading to the loss of CckA kinase and CtrA~P asymmetry in the early predivisional stage. (B) An eight-fold increase in DivK synthesis (ksyn = 0.4 min-1) induces PleC to become a kinase independent of DivJ. Hence, the swarmer stage of the cell cycle is circumvented.