The Internal Dynamics of Fibrinogen and Its Implications for Coagulation and Adsorption
Fig 4
Modelling of fibrinogen adsorption on mica and electrostatic analysis.
(a) The independent hinge model (blue line, χ2 = 1.55) and the general model (green line, χ2 = 1.0) for Fg flexibility upon adsorption are fitted to the experimental data (red histograms) of the distribution of the α angle in the tri-nodular structures observed by AFM measurements of Fg adsorbed on mica [7]. The error bars on the fit are equal to the square root of the expected count number N in each bin (i.e. assuming a poissonian distribution in each bin). The inset shows the definition of α on the simplified Fg model. (b) Typical bent conformation of the fibrinogen dimer with the electrostatic potential at D- and E-regions, highlighting the presence of a large negatively charged patch on one side of the surface of the D region. Drawn are the isosurfaces at ±26.7mV (blue/red).