BASiCS: Bayesian Analysis of Single-Cell Sequencing Data
Fig 9
(a) true versus estimated normalised expression level μi for each of the 46 ERCC spike-in genes (log-scale). Our estimations are gathered around the true values, except for lowly expressed technical genes, where the experiment suggests a small positive bias. Estimations according to [5] are highly correlated with the true values, however the true scale has not been recovered (not surprising as their method is not designed to estimate the right scale of the sj’s, see Fig 3). (b): Fig 8(a) superimposing (in black) estimated values for each of the 46 ERCC spike-in genes. (c) and (d): panels (c) and (d) of Fig 7 superposing (in black) estimated probabilities associated to each of the 46 ERCC spike-in genes. As expected, none of the spike-in genes were detected as HVG by our criteria. On the other hand, there is strong evidence in favour of being LVG for 21 of the technical genes (and some others are borderline according to our conservative criteria).