Dissecting the Calcium-Induced Differentiation of Human Primary Keratinocytes Stem Cells by Integrative and Structural Network Analyses
Fig 6
Combining expression classification with compatible (AND) and mutually exclusive (XOR) interaction types and summary of complex assembly motifs.
(A) Left panel, schematic representation of possible cases combining protein expression classification with surface interaction types: compatible (AND) and mutually exclusive (XOR) interactions. Discounting unresolved class, there are six possible cases, out of which three biologically meaningful ones were selected. Case 1: the hub protein is non-dynamically expressed while the attachment proteins are dynamic; case 2: all three interacting proteins are dynamic; and case 3: all three interacting proteins are non-dynamic. For the remaining cases, including unresolved genes, see S16 Fig Right panel shows percentages of protein interaction triplets analysed structurally by SAPIN for cases 1 to 3. Of protein triplets of case 1, 65% (n = 49) have XOR interactions compared to 35% (n = 27) AND, of case 2 61% (n = 128) have XOR interactions compared to 39% (n = 81) AND interactions and finally of case 3, 61% show AND interactions (n = 628) compared to 39% (n = 397) XOR. Statistical significance tested using Fisher test where * indicates p < 0.0001 and ** indicates p < 0.00001. Multiple testing correction was conducted with Bonferroni method resulting in all q < 0.005. For the remaining cases, including unresolved genes, see S19–S21 Figs (B) Summary of the complex assembly motifs proposed for the process of keratinocyte differentiation.