Accurate Encoding and Decoding by Single Cells: Amplitude Versus Frequency Modulation
Fig 1
Experimental evidence for amplitude and frequency modulation.
(A and B) Example data showing amplitude modulation from [10]. (A) Single-cell nuclear localization of Msn2 transcription factor in response to H2O2 stress as a function of time. The stimulus profile (input) is a step change applied at t = 0 (inset) which applies to all figure panels. (B) Average time trace for different concentrations of H2O2 stress. (C and D) Example data showing frequency modulation from [15]. (C) Single-cell nuclear localization of Crz1 in response to calcium stress as a function of time, showing bursts of Crz1. (D) The average frequency of bursts against calcium concentration, showing an increased frequency with increased concentration. (Inset) Burst duration distribution for low (blue bars) and high (red bars) concentration. Both histograms are well described by the Gamma distribution , with τb = 70s (black solid line), demonstrating that pulse duration is independent of calcium concentration. Experimental data in arbitrary units (AU) of fluorescence.