A Power-Law Dependence of Bacterial Invasion on Mammalian Host Receptors
Fig 1
Cell-cell variability in invasin-mediated bacterial uptake.
(A) Microscope image of phase and GFP signals from HeLa human cervical cancer cells co-cultured with E. coli harboring plasmids directing expression of (left) GFP only or (center, right) GFP and invasin (pSCT7Inv) at a multiplicity of infection (MOI) of 100 per mammalian cell for 60 minutes in the presence of gentamicin. Image on right is a magnification of boxed region. (B and C) Flow cytometry signals from E. coli (GFP/Invasin) incubated with (B) HeLa cells or (C) the indicated cell line as described in (A).