Deciphering Signaling Pathway Networks to Understand the Molecular Mechanisms of Metformin Action
Fig 2
Gene Set Enrichment Analysis (GSEA) enrichment score curves of metformin-induced probes in three treatments vs. treatment 1.
The four sets of probes of metformin treatments were obtained from the gene expression profiles from Connectivity Map. The three treatment instance IDs are 2, 3, and 4. The graphs on the top panels represent the ranked, non-redundant, and up-regulated probes in the second, third, and fourth treatment groups compared with probes in the first treatment group. The graphs on the bottom panels represent the ranked, non-redundant, and down-regulated probes in second, third, and fourth treatment groups compared with probes in the first treatment group. In each graph, probes on the far left (red) correlated with the most up-regulated probes in the treatment 1 and probes on the far right (blue) correlated with the most down-regulated probes in treatment 1. In each graph, the vertical black lines indicate the position of each of the probes of the studied probe set in the ordered, non-redundant data set. The green curve denotes the ES (enrichment score) curve, the running sum of the weighted enrichment score in GSEA.