Automated Processing of Imaging Data through Multi-tiered Classification of Biological Structures Illustrated Using Caenorhabditis elegans
Fig 5
First layer classification for detection of fluorescently labelled neuronal cells demonstrates generalizability of first layer features for particle shape classification.
a) Stereotypical positioning of the ASI neuron pair in the head of the worm. Many neuronal cells in the worm are organized as similar pairs near the pharynx. b) Bright field and fluorescent maximum intensity projection showing the appearance and positioning of fluorescently labelled ASI cells in the head of the worm. c) Preprocessing of raw fluorescent images showing binary image after Niblack thresholding (BW0) and initial filtration of the candidate set by size (BW1). d) First layer classification of fluorescently labeled neurons shows good generalizability of the first layer feature set developed for pharyngeal grinder detection for classification based on binary particle shape.