Disentangling the Complexity of HGF Signaling by Combining Qualitative and Quantitative Modeling
Fig 3
Experimental results and predictions by interaction graph models.
A) For each indicated protein, the fold change of the phosphorylation state measured by quantitative immunoblotting of two different experimental conditions is shown on a logarithmic scale at the indicated time points after HGF stimulation. Each row refers to one experiment; same experimental conditions are grouped with magenta lines. B) The discretization (left panel) is based on Fig 3A and additional SOS1 measurements (S1A Fig). A slash shows that no measurements were taken or that the response was not conclusive. In the middle and right panel, predictions from the core model and, exemplary, from the identified substructure 1 (Fig 4) are shown. Arrow pointing up/down: the inhibition can only cause an increased/decreased activation of the measured protein. Bullet point: the inhibition does not affect the measured protein. Combined up/down arrow and bullet point: the model does not restrict the response to the inhibition.