Site-Specific Phosphorylation of VEGFR2 Is Mediated by Receptor Trafficking: Insights from a Computational Model
Fig 6
Increased total VEGFR2 activation with immobilized VEGF is driven by the change in surface VEGFR2.
All panels show area under the curve (AUC), a measure of total VEGFR2 activation, for the first 60 minutes after stimulation with soluble (Vs- blue) or immobilized (Vb- green) VEGF at a concentration of 2 ng/mL. AUCs are shown for surface VEGFR2 (A-D), Rab4/5 VEGFR2 (E-H), and total VEGFR2 (I-L). Activation on any considered tyrosine residue (pR2, 1st column), Y1175 (2nd column), and Y1214 (3rd column) are compared. The last column shows the AUC for the curve pY1214/pY1175 (total pY1214/pY1175 curves shown in Fig 8F and 8G) for surface VEGFR2 (D), Rab4/5 VEGFR2 (H), and total VEGFR2 (L). Note that the difference in total pY1214/pY1175 for Vs and Vb emerges from the altered VEGFR2 distribution, not altered pY1214/pY1175 in each subcellular location.