< Back to Article

Segmentation and Tracking of Adherens Junctions in 3D for the Analysis of Epithelial Tissue Morphogenesis

Fig 3

Analysis of Drosophila notum morphogenesis.

A) A Maximum intensity projection of an image stack through the mid-scutum marked with E-cad∷GFP to highlight cell outlines. Anterior to the right. B) Maximum intensity projection of the output of the filters employed to detect the AJs (green) and AJ vertices (red). C) AJ graph (green) and Cell graph (blue) symbolically represent the cell outlines and their connectivity, respectively. D) Projection of the 3D centroid trajectories recovered after tracking the motion of cells. Note that motion of cell centroid varies depending on the position of the cell across the tissue. E) Evolution of cell strain rate parameters ($\frac{\partial \stackrel{.}{x}}{\partial x}$, $\frac{\partial \stackrel{.}{x}}{\partial y}$, $\frac{\partial \stackrel{.}{y}}{\partial x}$, $\frac{\partial \stackrel{.}{y}}{\partial y}$) computed from Kalman smoothed trajectories of cell centroids over time. F) Mean velocities (${\stackrel{.}{x}}_0$, ${\stackrel{.}{y}}_0$) of the cell centroid trajectories over time. Time evolution of G) expansion coefficient ℰ, and H) rotation coefficient θ. See text for further detail.

doi: https://doi.org/10.1371/journal.pcbi.1004124.g003