A Theoretical Justification for Single Molecule Peptide Sequencing
Fig 6
Monte Carlo sampling reveals the confidence with which fluorosequences can be attributed to specific source proteins.
(A) and (B) represent two example fluorosequences, illustrating opposite extremes in terms of the number of proteins capable of yielding each sequence. In (A), the frequencies with which rival source proteins yield fluorosequence “xxxxExxKxK” in the Monte Carlo simulations indicates low confidence in attributing that fluorosequence to any one protein. In (B), a single protein is by far the most likely source of fluorosequence “EEEEExxKxK”. (X-axes represent incomplete lists of proteins, ordered by the frequencyies with which they are observed to generate the given fluorosequence in the simulations.)