Data-Driven Prediction and Design of bZIP Coiled-Coil Interactions
Fig 3
Specificity profiles of four designs tested at 37°C.
The designed binders interact strongly with their targets as rhodamine-labeled peptides (bold red circles). Thin red circles show interactions with other bZIPs in the same family as the target. The ATF5-d1 design bound more tightly to ATF4 (thin red circle) than to ATF5. The designed proteins do not form strong homodimers (black bars), and there are large specificity gaps between the design/target interactions and design/off-target interactions (white and grey bars, colored according to target-off-target sequence identity at a, d, e and g positions). All Kd values are listed in S5–S12 Tables.