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The Thalidomide-Binding Domain of Cereblon Defines the CULT Domain Family and Is a New Member of the β-Tent Fold

Figure 8

Comparison of the substrate-binding sites in CULT and MsrB.

The superimposition shows the thalidomide binding sites in the CULT domain structures (M. gryphiswaldense MGR_0879, G. gallus 4CI2, M. musculus 4TZC) in gray and the methionine sulfoxide binding site of MsrB (X. campestris 3HCI) in red. The ligands are colored cyan (thalidomide) and marine blue ((2S)-2-(acetylamino)-N-methyl-4-[(R)-methylsulfinyl]butanamide). Residue numbers are provided for the M. gryphiswaldense structure in gray, and for MsrB in red.

Figure 8

doi: https://doi.org/10.1371/journal.pcbi.1004023.g008