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The Thalidomide-Binding Domain of Cereblon Defines the CULT Domain Family and Is a New Member of the β-Tent Fold

Figure 5

Cluster map of CULT domain homologs.

The image is a cross-eyed stereo view and shows that the five domain families occupy the vertices of an approximately equilateral pyramid. For this map, we searched the nr database at NCBI with PSI-Blast, using the CULT domain protein MGR_0879 of M. gryphiswaldense as a query. After six iterations, we extracted all proteins above the cutoff of E = 0.005 and clustered them in CLANS using their all-against-all pairwise similarities as measured by BLAST P-values. Clustering was done to equilibrium in 3D at a P-value cutoff of 1e-5 using default settings.

Figure 5

doi: https://doi.org/10.1371/journal.pcbi.1004023.g005