Dissecting Dynamic Genetic Variation That Controls Temporal Gene Response in Yeast
Figure 6
A genetic variant acting on the timing of response of the poor nitrogen-source degradation pathway (module no. 5-II).
(A) The genomic interval underlying module no. 5-II residing in Chr2: 533–562 kb. Shown are DyVER scores (y-axis) across the genomic positions in chromosome 2 (x-axis) for seven associated genes (color coded; the module includes only those six genes that cross the FDR 6% threshold). Positions of two potential causal variants, RPB5 and CNS1, are marked below. (B) Genetic effects, relative to non-stimulated genetic effects (y-axis, log-scaled) for different associated genes from A (color coded) at six time points (x-axis). The plot depicts the short impulse of high genetic effect in all associated genes. (C) Module genes, in the context of the poor nitrogen-source degradation pathway. Enzymes are shown as color-coded rectangles (bold-pink/module genes, pink/associated genes, white/non-associated genes). The pathways show the uptake of poor nitrogen sources (allantoate, allantoin, and GABA) and their degradation into ammonium. (D) A representative gene. Expression profiles (left) and genetic effects (right, y-axis) of DAL80, a gene in module no 5-II, during response to rapamycin (x-axis). Shown as in Fig. 4B but using a marker near the RPB5 gene (marked in A).