Structure, Dynamics and Implied Gating Mechanism of a Human Cyclic Nucleotide-Gated Channel
Figure 3
Evolutionary coupling (EC) calculations support the model.
Contact maps of top-ranked predicted ECs (red) overlaid on monomer (light grey) and intermonomer (dark grey) contacts from (A) the model structure of the TM domain of CNGA3; (B) the model structure of the cytosolic domain of CNGA3. The insets show the contact maps of alternative CNGA3 model structures; the orange arrows point to evolutionary couplings between amino acid pairs that are not in contact in the alternative models, but are in contact in our final model. Clearly, the overlay of the ECs with the contact map is far better for the chosen model structure than for the alternatives.