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Molecular Dynamics Study of the Opening Mechanism for DNA Polymerase I

Figure 7

An overlay of the DNA polymerase fingers subdomain for the 3HP6 crystal structure (blue ribbons) and the observed 1LV5 intermediate state (yellow ribbons) characterized by the Arg703-Glu562 salt bridge.

Although the end of the O-helix for both structures is near the same location, the α-helices in the fingers domain are clearly different resulting in the 4.3 Å RMSD between the 1LV5 intermediate and the 3HP6 crystal structure.

Figure 7

doi: https://doi.org/10.1371/journal.pcbi.1003961.g007