Impact of Dimensionality and Network Disruption on Microrheology of Cancer Cells in 3D Environments
Figure 2
Intracellular microrheology of a single cell in 3D in a microfluidic device.
a) Fluorescence image of an MDA-MB-231 cell in 2.5 mg/mL collagen. Actin is green and mitochondria are red. b) 1D MSDs in the two orthogonal directions with the maximum ratio in displacement fluctuations. Blue is the average of the MSDs in the directions of maximum fluctuation and green is the average of the MSDs in the directions of minimum fluctuation. Maximum and minimum directions are determined on a particle by particle basis. Yellow curves represent the 1D MSDs of each individual mitochondrion tracked. c,d) The complex shear modulus G in the directions with maximum (c) and minimum (d) fluctuations. Green is the elastic modulus and red is the loss modulus. e,f) Spatial plots of the log of the magnitude of the shear modulus G in the direction of (e) maximum and (f) minimum fluctuations at 10 Hz. Arrows point in the directions with the maximum (e) or minimum (f) fluctuations. Colors represent the magnitudes of log|G|. This experiment was performed at ambient conditions. The scale bar is 20 µm.