Estimating Relative Changes of Metabolic Fluxes
Figure 5
Analysis of experimental data.
(a) The diagram of glycolysis and its two branching pathways used in the analysis, where dashed squares (e.g., BPG) represent missing metabolites, dashed arrows represent missing pathways, and dashed rectangles containing solid squares represent undistinguished metabolites. Abbreviations for metabolites: GLU: glucose; G6P: glucose-6-phosphate; F6P: fructose-6-phosphate; FBP: fructose-1,6-bisphosphate; DHAP: dihydroxyacetone phosphate; GAP: glyceraldehyde-3-phosphate; BPG: 1,3-biphosphglycerate; 3PG: 3-phosphoglycerate; 2PG: 2-phosphoglycerate; PEP: phosphoenolpyruvate; PYR: pyruvate; PGL: 6-phosphogluconolactone; 6PG: phosphogluconate; R5P: the pool of ribose 5-phosphate, ribulose 5-phosphate and xylulose 5-phosphate; PHP: phosphohydroxypyruvate; 3PS: 3-phosphoserine; SER: serine; GLY: glycine. (b) An exemplary plot of the data of a metabolite and its fit. Plots of all metabolites and their fits can be found in Figure S3. (c) Histograms of 's as generated by sampling the corresponding posterior distributions in a way detailed in the Methods. Glycolysis flux refers to
in the diagram, PPP flux
, and serine synthesis flux
. The three histograms for each flux correspond to three different modeling choices described in the text.