Live Imaging-Based Model Selection Reveals Periodic Regulation of the Stochastic G1/S Phase Transition in Vertebrate Axial Development
Figure 8
Summary of methodology and findings.
(A) Schematic summary of our methodology. Our methodological approach consisted of three successive processes: 1) live imaging and image processing, 2) model establishment and 3) model selection. Each process was further divided into sub-processes, as noted in each box. (B) Schematic illustration of the regulatory mode of the G1/S transition in the developing notochord. (B-i, B-ii) Once the G1/S transition window was established between populations of anterior green (S) and posterior (G1) red cells, the G1 cells in the window stochastically transitioned into the S phase (B-i), after which the window was finally filled with green (S) cells (B-ii). (B-iii, B-iv) The next transition window was established posteriorly adjacent to the previous window. As observed in the previous cycle, an increasing number of green (S) cells filled the window as time progressed (B-iii), until the window was finally filled with green cells (B-iv).