Glycolysis Is Governed by Growth Regime and Simple Enzyme Regulation in Adherent MDCK Cells
Figure 8
Prediction of glycolytic metabolite pools during cultivation of adherent MDCK cells in DMEM with 2.5 mmol L−1 extracellular glucose.
Glucose 6-phosphate (A), fructose 6-phosphate (B), fructose 1,6-bisphosphate (C), 3-phosphoglyceric acid (D) and phosphoenolpyruvate (E) concentrations during MDCK cell cultivations in 6-well plates and DMEM medium with 2.5 mmol L−1 extracellular glucose. Data (◊) and error bars represent mean and standard deviation of three wells. Dashed lines are the limit of quantification (LOQ; data below LOQ marked in grey). Lines represent the model prediction based on the modifications of the cell growth model described in the supporting information 4 and the parameters in Table 1 and Table 2. The intermediate growth phase (95%–5% proliferating cells) is indicated as grey bar.