Glycolysis Is Governed by Growth Regime and Simple Enzyme Regulation in Adherent MDCK Cells
Figure 5
Intracellular metabolite pools of pentose phosphate pathway and glycogenesis during adherent MDCK cell cultivation.
Ribose 5-phosphate (A–C) and uridyl diphosphate glucose (D–F) concentrations of three independent MDCK cell cultivations (Cult1 ▵, Cult2 □, Cult3 ○) in 6-well plates and GMEM-Z. Data and error bars represent mean and standard deviation of three wells, respectively. Dashed lines are the limit of quantification (LOQ; data below LOQ marked in grey). Lines represent the respective simulation result based on the experiment-specific parameters in Table 1 and parameters in Table 2. The intermediate growth phase (95%–5% proliferating cells) is indicated as grey bar for the respective cultivation.