Glycolysis Is Governed by Growth Regime and Simple Enzyme Regulation in Adherent MDCK Cells
Figure 2
Metabolites pools of glycolysis during adherent MDCK cell cultivation.
Glucose 6-phosphate (A–C), fructose 6-phosphate (D–F), fructose 1,6-bisphosphate (G–I), 3-phosphoglyceric acid (J–L) and phosphoenolpyruvate (M–O) concentrations in three independent MDCK cell cultivations (Cult1 ▵, Cult2 □, Cult3 ○) in 6-well plates and GMEM-Z. Data and error bars represent mean and standard deviation of three wells. Dashed lines are the limit of quantification (LOQ; data below LOQ marked in grey). Lines represent the respective simulation result based on the experiment-specific parameters in Table 1 and parameters in Table 2. The intermediate growth phase (95%–5% proliferating cells) is indicated as grey bar for the respective cultivation.