Glycolysis Is Governed by Growth Regime and Simple Enzyme Regulation in Adherent MDCK Cells
Figure 1
Scheme of glycolysis model (B) and its link to the segregated cell growth model (A) established previously [23].
Green boxes represent metabolite pools that were quantified experimentally while white ones were not measured. Enzymes are shown as ellipses with blue background if the maximum enzyme activity was measured in vitro and with white background otherwise. Reactions and their directions are shown as arrows. Dashed arrows represent allosteric regulation of enzymes by metabolites. The activity of the enzymes relative to the highest activity in glycolysis (see legend) is attached to the corresponding reactions/enzymes and expressed by colored bars (blue: cell growth at 24 h of Cult1; green: cell maintenance at 100 h of Cult1; orange: limitation at 6 min). Absolute flux rates (unit: mmol L−1 min−1) are given next to the bars. GLCx extracellular glucose; GLC glucose; G6P glucose 6-phosphate; UGLC uridyl diphosphate glucose; R5P ribose 5-phosphate; PPP pentose phosphate pathway; F6P fructose 6-phosphate; F16BP fructose 1,6-bisphosphate; 3PG 3-phosphoglyceric acid; PEP phosphoenol pyruvate; HK hexokinase; UT UTP-glucose-1-phosphate uridylyltransferase; G6PDH glucose 6-phosphate dehydrogenase; GPI glucose phosphate isomerase; ALD aldolase; ENO enolase; PK pyruvate kinase.