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Genome-Scale Metabolic Network Validation of Shewanella oneidensis Using Transposon Insertion Frequency Analysis

Figure 4

Insertion frequency within genes.

Genes were evaluated in 0.2% gene increments. No insertion preference was observed in the complete gene population (A). The same analysis was performed (1% increments) after essential (red) and nonessential (green) gene identification (B). The last ∼2% at the 3′ end of essential genes were inserted more frequently.

Figure 4

doi: https://doi.org/10.1371/journal.pcbi.1003848.g004