Genome-Scale Metabolic Network Validation of Shewanella oneidensis Using Transposon Insertion Frequency Analysis
Figure 1
Evaluation of transposon insertion bias.
(A) Scatter plot of the genome location dependent insertion probability. Each data point represents a genomic window of 20,000 nucleotides, which was shifted by 2,000 nucleotides for each consecutive evaluation. The insertion probability gradually decreased from the origin of replication. The solid line is a best fit of ax2+b|x|+c, where a, b and c are equal to 0.0032, 0.0081 and 0.1615, respectively, and x is the location in the genome. (B) Boxplot of flanking sequence dependent insertion probability distribution showing that several motifs contained significantly more insertions than others. (C) Relative occurrence of nucleotides in the two flanking positions of inserted TA sites.