PTEN Hopping on the Cell Membrane Is Regulated via a Positively-Charged C2 Domain
Figure 5
Rebinding probability analysis.
(A) Rebinding probabilities of wild-type PTEN, PTEN mutants and Latrunculin A treated PTEN4 mutant. The probability that a molecule rebinds to the membrane between 0.45 and 2 µm from the vanished position and within 133 msec after the vanished time was calculated using Eq. 9. * and ** indicate p<0.05 and p<0. 01 obtained by Student's t-test, respectively. (B) Spatial distribution of rebinding probabilities of wild-type PTEN (orange) and PTEN4 (blue). The spatial distributions were obtained between 0 and 33 msec (upper) and 33 and 66 msec (lower) after spots vanished (right panels). The bin range is 0.05 µm. Typical images of hopping molecules that rebind between 0 and 33 msec (upper) and 33 and 66 msec (lower) are shown (left panels). The minimum limit of the analysis is 0.45 µm from the previous position (white dotted circles). Trajectories before hopping are shown in yellow. (C) Temporal changes in rebinding probability of wild-type PTEN (upper) and PTEN4 (lower). Data are mean +/− SD. (D) Typical images showing a succession of jumps and the corresponding trajectory (yellow). Numbers are time in milliseconds. Scale bar, 1 µm. (E) Hopping lifetimes of wild-type PTEN, PTEN4 and PTEN7.