PTEN Hopping on the Cell Membrane Is Regulated via a Positively-Charged C2 Domain
Figure 3
PTEN molecules hopping on the cell membrane.
(A, B) Hopping occurred between the second and third frames. (C, D) Hopping occurred within the second frame. (E, F) Blinking. (A, C, E) Images of single molecules. (B, D, F) Fluorescence intensity profiles along the long axis of the rectangles shown in A, C and E. (G) Cloud-like fluorescence (dotted rectangle at t = 66 msec) of hopping molecules. Numbers in the upper right of each panel are time in milliseconds. Scale bar, 1 µm. (H) Trajectories of molecules showing hopping, blinking and lateral diffusion. Thick lines show hopping displacements. Colors in the trajectories indicate the moments of hopping shown in (B) and (D).