Construction and Validation of a Regulatory Network for Pluripotency and Self-Renewal of Mouse Embryonic Stem Cells
Figure 5
Comparison between computational in silico and experimental knockdowns followed by expression measurements.
(A) Design of in silico and experimental knockdowns. (B) Simulation results of computational knockdowns. Red represents up-regulation, blue represents down-regulation and white represents no change with regards to the unperturbed condition. (C) Results of experimental knockdowns followed by mRNA measurements of all network nodes, where each experiment was repeated twice. Colorbar illustrates log-scaled fold-change. Red represents up-regulation and blue represents down-regulation with regard to the unperturbed condition. (D) Comparison of computational and experimental knockdowns (see Text S1). Colors correspond to discordance score defined in the objective function in the supplementary material. Concordant results are colored in yellow while discordant results are colored in blue with gradients representing the degree of (dis)agreeability.