Construction and Validation of a Regulatory Network for Pluripotency and Self-Renewal of Mouse Embryonic Stem Cells
Figure 3
(A-B) Plots of normalized Ct-values partitioned into two clusters.
(C–D) Hierarchical clustering of gene expression in single mESCs using normalized Ct-values from RT-PCR data measuring levels of 15 pluripotency genes (orange) and 15 lineage-specific genes (purple) in 96 cells cultured in serum/LIF (C) and in 2i/LIF (D). The x-axis represents individual cells and the y-axis represents genes. Yellow represents ‘high’ and blue represents ‘low’ levels of gene expression. (E–F) Association of 30 genes based on gene expression in single mESCs cultured in serum/LIF or 2i/LIF. Numeric values in the color bars represent the distance score calculated as 1 – Pearson-Correlation Coefficient (PCC). Average-linkage, Euclidean-distance-based hierarchical clustering was performed the gene expression data.