Construction and Validation of a Regulatory Network for Pluripotency and Self-Renewal of Mouse Embryonic Stem Cells
Figure 2
30 mRNA gene expression measurements in 96 single mESCs cell using the Fluidgm single cell microfluidic device.
Histograms of gene expression Ct-values from RT-PCR data collected from single mESCs in +serum/LIF (serum/LIF, blue) and -serum/2i/LIF (2i/LIF, red). Ct-values were normalized using the housekeeping gene Gapdh. x-axis represents -ΔCt values and y-axis represents the percentage of total cells. Alpha blending is used to show the results from the other condition for clearer visual comparison.