Random Migration and Signal Integration Promote Rapid and Robust T Cell Recruitment
Figure 3
Quantifying the contribution of signal integration and probabilistic priming to in vivo T cell retention kinetics.
Starting with a general model that accounts for both signal integration and probabilistic priming (top row), we disable each model component separately by setting its parameter to a constant value. Specifically, fixing the success rate to 100% disables probabilistic priming by enforcing that, during every contact, a (possibly small) cognate signal is transmitted (middle row). Similarly, fixing the required contacts to 1 disables signal integration (bottom row). The best fit of each model to retention timecourses obtained from the two-photon data for the M-peptide are shown. In contrast to the coarser analysis of Figure 2, we now consider each experiment separately (filled circles, open circles, and crosses) and use a higher time resolution (details in Methods). The size of each data symbol is proportional to the total duration of all tracks it represents. The characteristic switch-like pattern of signal integration is clearly present in the 200 pM dose (right panels), which the purely probabilistic model fails to fit.