Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration
Figure 7
Applying the same analysis and comparing the results to DA3 cells yielded similar directionality (A, C), and coordination (B, D) for the control case and in response to HGF/SF. (E) HGF/SF enhances persistent migration of MDCK cells (left – mean persistence as a function of the distance from the wound edge, right – full distribution of trajectories' persistent migration), accumulated over all experiments (N = 5 for HGF/SF treated cells, N = 5 for control cells). (F–G) Association between the waves, accumulated over all experiments (N = 5 for HGF/SF treated cells, N = 5 for control cells). (F) Acceleration and directionality, optimal delay was 217 minutes. Inset: association when considering the optimal delay. (G) Directionality and coordination. All phenomena observed in DA3 cells were evident also in MDCK cells, but with a lesser extent. We hypothesize that the tighter adhesions between MDCK cells [53] reduces the above mentioned phenomena by limiting efficient acceleration of cells in the wound's direction.