Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration
Figure 4
HGF/SF-induced wave of directionality.
(A, C) Spatiotemporal maps (kymographs) of the average directionality of DA3 tumor cells, in response to HGF/SF treatment (C), in comparison with the control results (A). The x-axis represents the time measured in minutes and the y-axis represents the distance from the wound edge in microns (see the text and Figure 2). (B, D) The average directionality for four 100-minute time intervals of the spatiotemporal maps in (A) and (C), respectively. (E–F) HGF/SF enhances persistent migration of DA3 cells. (E) Average persistent migration as function of distance from the wound edge, with and without HGF/SF. In response to HGF/SF, cells migrate with higher persistence. Each treatment plot was composed of 3,000–4,000 distinct trajectories extracted throughout Phase 1. (F) Full distributions of trajectories' persistence, accumulated over all experiments.