Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration
Figure 3
HGF/SF-induced waves of acceleration and stretching.
(A–D) Spatiotemporal maps (kymographs) of acceleration (A and C) and strain rate (B and D), calculated for each agent as explained in the text and in Supporting Text SI4 in Text S1. The x-axis represents the time in minutes and the y-axis represents the distance from the wound edge in microns. Each element (t,d) in the map shows the average acceleration (in A and C) and strain rate (in B and D) measured at time (t) for all the agents of a layer of width Δd = 12.4 µm (10 pixels), located at a distance (d) from the wound edge, over a time interval Δt = 14.5 minutes (1 frame). (E) and (F): the average coordination for four 100-minute time intervals of the spatiotemporal maps in (C) and (D), respectively. These figures illustrate the highly coinciding waves of increasing motility (acceleration) and stretching (strain rate).