The Long and Viscous Road: Uncovering Nuclear Diffusion Barriers in Closed Mitosis
Figure 6
Estimated number n of proteins at the ring constraining lateral diffusion in EA nuclei.
Average deviations (in percentages) of stochastic simulations from the experimental mean for each experimental time step. Mother and daughter lobe deviations are calculated as the absolute value of the difference between simulations and FLIP experiments. The average deviation is the mean of both nuclear lobes' deviations over time. The best fit (i.e. minimal deviation) is shown as a continuous line. For NPC data, we assumed the specialised lipid domain lies at both the ONM and INM, whereas the protein ring is present at the ONM only. Effective diffusion coefficients were fixed as in Table S2 and we fixed Pout = 100% in all cases. Pin values were fixed as in the homogeneous domain scenario in LA (Table 1). For Nsg1-GFP, ns stands for the number of septin proteins in a double ring arrangement.