The Long and Viscous Road: Uncovering Nuclear Diffusion Barriers in Closed Mitosis
Figure 4
Barrier permeabilities of different specialised lipid domain configurations and for maker proteins at the ONM and INM.
The transmission coefficient of the barrier depends directly on the partition coefficient and the diffusion rate within the domain, and is inversely dependent on its thickness (see Methods). The error bars are associated to the heterogeneous distribution of surface areas (21 nuclei in EA and 34 in LA). For the single ring configuration in LA, a ring of 100 nm wide was assumed for diffusion of NPCs (asterisks). For the NPC, we considered both scenarios where the domain lies only at the ONM or at both ONM and INM. Effective diffusion coefficients were fixed as in Table S2 and we fixed Pout = 100% in all cases. The corresponding Pin values are shown in Table 1.