CeleST: Computer Vision Software for Quantitative Analysis of C. elegans Swim Behavior Reveals Novel Features of Locomotion
Figure 2
Examples of ten CeleST measure outputs for an individual C. elegans swim trial.
All measures reflect analysis of a 30/sec, with C–G calculated from analysis of radius of curvature over 12 body segments (curvature plot vs. time example is in B). For C–G and M–O, instantaneous values are plotted in black; the median value for each swim is drawn in red, and the 10–90 percentile range of values is shown in gray; median and range over the 30 s trial are listed on the right. Note that although this panel demonstrates analysis of measurements of a single animal swimming, the CeleST program score multiple animals simultaneously and can compile data from thousands of individual swim trials (examples in Figure 3). A, Scored animal at three indicated time points in the video, with the curvature measure superimposed on the body; color key shown in B. B, Curvature heat map of an individual swim trial. Map is of curvature at a given body point (Y axis) as a function of time (X axis), with head curvature score at the top and tail curvature at the bottom on the Y axis, deep bend in one direction dark blue, and in the other direction dark red. Lines indicate posture of the animal depicted at that time point in panel A. Note that the posture of an individual at any point in time could be reconstructed from the measure of curvature over body position. Further details on each parameter measurement C–O are given in Text S1. Informative videos (Videos S2 01–10) that feature extreme examples for each measure can be found on http://celest.mbb.rutgers.edu.