CCAST: A Model-Based Gating Strategy to Isolate Homogeneous Subpopulations in a Heterogeneous Population of Single Cells
Figure 5
Signaling behavior in B-cell subtypes for CCAST vs manual gating strategy.
A Heatmap of BCR, IFNa, FTL3, IL3, IL7, and SCF induced intracellular signaling responses in 5 B-cell CCAST-derived subtypes, compared with those of an unstimulated control. B Heatmap of BCR, IFNa, FTL3, IL3, IL7, and SCF induced intracellular signaling responses in the five B-cell subtypes obtained from the manual gates in Bendall et al. [9], compared with those of an unstimulated control. The higher difference implies a stronger signal in the CCAST-derived cell type compared to the manually gated cell type.