CCAST: A Model-Based Gating Strategy to Isolate Homogeneous Subpopulations in a Heterogeneous Population of Single Cells
Figure 4
CCAST applied to single cell analysis of B-cells.
A Silhouette plot showing evidence of 5 B-cell types. B CCAST gating strategy for B-cell types based on CD45, CD34, CD38, and CD123 markers using 3 levels of gating. The estimated ranges for the split point variables are provided at each node. Note Celltype 3 is distributed across three gated populations. C Cross classification heatmap of manually gated and CCAST predicted B-cell types indicates strong evidence that the most abundant Mature CD38low B-cells comprise a mixture of other subtypes (Celltype 2 and 4). D Heatmaps show evidence of the two derived distinct mature B-cell states corresponding to Celltypes 2 and 4 based mainly on CD123 (label highlighted in red).