Quantifying the Length and Variance of the Eukaryotic Cell Cycle Phases by a Stochastic Model and Dual Nucleoside Pulse Labelling
Figure 7
Effect of cell death and completion time distribution on parameter estimates.
A: Comparison of analytical predictions (lines, Eq. 29) with simulated BrdU labelling experiment (squares). Cell death is assumed to occur exclusively during S phase with probability 0 (red) and 0.3 (blue) respectively. Only the population is considered. Parameters:
units are hours. B: Difference between Eq. 29 (accounting for cell death) and Eq. 24 (neglecting cell death) at time
h (see dashed line in A), as a function of
C: BrdU labelling experiments were simulated assuming gamma distributed phase completion times (red curve, graphs on left column) and cell death during S phase with probability
and
(green curve, graphs on left column). The effective completion time
(gray density plot, left column), the population growth (middle column) and the estimation of the mean and the standard deviation of
are shown for both cases. Approximate confidence intervals for the estimates are computed as 1.96 times the standard error. Even though
and the population growth are strongly influenced by the value of
both
and the estimates extracted from
are barely affected. The dashed lines in the middle column indicate the time of the first pulse, which was chosen such that the average population was similar in both scenarios. Parameters for gamma distributed completion time distribution of the three phases: shape:
scale:
delay: