Quantifying the Length and Variance of the Eukaryotic Cell Cycle Phases by a Stochastic Model and Dual Nucleoside Pulse Labelling
Figure 3
Model based parameter estimation.
A: Best fit of the model predictions (lines) to experimentally determined cell fractions after BrdU pulse labelling (dots). U87: In vitro cultured U87 human glioblastoma cancer cell line (three replicates). V79: In vitro cultured V79 Chinese hamster cells (single replicate) (courtesy G. Wilson). Best fit parameter values used to compute model predictions (U87: V79:
units are hours). B: Approximate ML regions for the parameters
and
associated to each phase (gray:
red:
green:
). C: Bayesian bi-variate 99%-credibility regions for the parameters
and
for each phase. Arrows indicate point estimates and the dashed lines delineate the information that could have been gained in our thought experiment under noise-free conditions from two support points, one at
and a second at
. The U87 data set was generated as described in the Experimental Methods section. The V79 data set was a kind gift of G. Wilson.