Quantifying the Length and Variance of the Eukaryotic Cell Cycle Phases by a Stochastic Model and Dual Nucleoside Pulse Labelling
Figure 2
DAPI-BrdU pulse-chase labelling FACS data.
Samples taken at several time points after pulse labelling proliferating U87 human glioblastoma cells with The four gated populations are
and
which are defined precisely in the main text. Briefly, the subscript indicates the phase at the instant of labelling, while the superscripts ‘u’, ‘lu’ and ‘ld’ refers to cells ‘unlabelled’, ‘labelled and undivided’ and ‘labelled and divided’, respectively. The data was generated as described in the Experimental Methods section.