Feedback Signals in Myelodysplastic Syndromes: Increased Self-Renewal of the Malignant Clone Suppresses Normal Hematopoiesis
Figure 5
CFU-Frequency upon stimulation with MDS serum.
(A) CD34+ HPCs from cord blood were cultured with 10% serum supplements of individual MDS patients or controls. After seven days, cells were re-seeded in methylcellulose medium and after two weeks, the numbers of erythrocyte (BFU-E and CFU-E), granulocyte (CFU-G), macrophage (CFU-M) and combined (CFU-GM and CFU-GEMM) colonies were counted. (B) Erythropoietin (EPO) concentration was significantly higher in MDS-derived serum samples. (C) The growth promoting effect on CD34+ cells was then plotted against the EPO concentration. CFSE intensity was normalized to healthy controls of the corresponding experiment. Error bars represent SD (*p<0.05, **p<0.01, ***p<0.001).