Coupling of Lever Arm Swing and Biased Brownian Motion in Actomyosin
Figure 8
Importance of conformational flexibility and electrostatic interactions for the global gradient of the landscape.
(A) Simulated free-energy landscapes in the A.Mclosed state assuming the structurally fluctuating parts of myosin and the N-terminus of actin to be disordered without guidance of the Gō-like potentials (black solid), assuming the structurally fluctuating parts of myosin to be disordered but the N-terminus of actin to fluctuate around the estimated conformation by following the Gō-like potential (black dashed), assuming myosin to fluctuate around the estimated conformation and the N-terminus of actin to be disordered (red solid), and assuming loop 2 and loop 3 of myosin and the N-terminus of actin to be disordered with other parts fluctuating around the estimated conformation by following the Gō-like potential (red dashed). (B) Simulated free-energy landscapes in the A.Mclosed state for 25 mM KCl solution (Debye length 1.9 nm, black line) and for 100 mM KCl solution (Debye length 0.95 nm, red line).